Body Fluid Culture, Sterile

Body Fluid Culture, Sterile

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Synonyms

Culture, Body Fluid, Sterile, Routine; Sterile Body Fluid Culture, peritoneal, pericardial, plural, ascitic, synovial, etc.

Aim of the test

Isolate and identify pathogenic organisms from normally sterile body fluids and perform sensitivity test

Body Fluid Culture, Sterile

Types of specimen

Aseptically aspirated body fluid (e.g., synovial, peritoneal fluid).

Criteria of specimen rejection

Inappropriate specimen transport device; mislabeled specimen; unlabeled specimen; specimen received after prolonged delay (usually more than two hour); specimen received in expired transport media

Infection of sterile body fluid: all body fluid are sterile

Common pathogenic of precarditis and myocarditis

  • Mycoplasma pneumoniae
  • Chlamydia trachomatis
  • Mycobacterium tuberculosis
  • Staphylococcus aureus
  • Streptococcus pneumoniae
  • Enterobacteriacae and other gram negative bacilli

Pleural fluid

  • Staphylococcus aureus
  • Pseudomonas spp.
  • Enterobacteriacae
  • Streptococcus pneumonia
  • Anaerobic bacteria
  • Mycobacterium tuberculosis
  • Haemophilus influenzae
  • Actinomyces spp.

Bones and joints

  • Staphylococcus aureus
  • Streptococcus pyogenes
  • Haemophilus influenzae
  • Enterobacteriacae
  • Mycobacterium spp
  • Neisseria gonorrheae
  • Streptococcus pneumonia

Peritoneal fluid

  • Streptococcus pneumoniae
  • Group A streptococci
  • Enterobacteriacae
  • Other gram negative bacilli
  • Staphylococci
  • Neisseria gonorrheae
  • Chlamydia trachomatis

Pre specimen processing

Patient preparing: Swab skin over the site of puncture with 2% tincture of iodine in concentriccircles.


Note: Iodine should remain in contact with skin for at least 1 minute prior to puncture to ensure complete antiseps is. Following puncture, 70% alcohol is used to remove iodine from skin.

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Specimen collection

Contamination with normal flora from skin, rectum, vaginal tract, or other body surfaces should be avoided. Indicate the specific source and pertinent clinical history on the request form.

Who will collect the specimen: Physician

Quantity of specimen: 1-5 mL is adequate.

Time relapse before processing the sample

Body fluids should be treated as CSF specimens and should processed immediately.

Storage: Maintain specimen at room temperature. Do not refrigerate.

Specimen processing

Media

1. Blood Agar (2 plates) 2. Chocolate Agar, 3. MacConkey Agar 4. Thioglycollate broth

Post specimen processingInterfering factors:

Patient on antibiotic therapy. Improper sample collection.

Result reporting: Report Gram stain finding as an initial report. Report the isolated pathogen and its sensitivity pattern as a final report.

Turnaround time: Gram stain and wet mount results should be available 1 hour after specimen receipt. Isolation of a possible pathogen can be expected after 2-3 days. Negative culture will be reported out 1-2 days after the receipt of the specimen.

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